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dc.contributor.advisorMerien, Fabrice
dc.contributor.authorZhang, Ke
dc.date.accessioned2018-05-23T03:52:58Z
dc.date.available2018-05-23T03:52:58Z
dc.date.copyright2018
dc.identifier.urihttp://hdl.handle.net/10292/11565
dc.description.abstractBackground Leptospirosis is a worldwide zoonosis that is endemic in tropical areas. The species Leptospira interrogans is the primary agent in human infections, but other pathogenic species, such as L. kirschner and L. borgpetersenii, are also associated with human leptospirosis. Methods and Findings In this study, an unsupervised high resolution melting (HRM) analysis of the products that were amplified with five pairs of primers lfb1 F/R, G1/G2, VNTR-4Bis, VNTR-Lb4 and VNTR-Lb5 facilitated an accurate species classfication of Leptospira reference strains from New Caledonia Institute Pastéur. Next, the genotypes at the subspecies level was identificated by using method with LightCycler®480 instrument and the High Resolution Melting Master kit (04909631001). LightCycler®480 Gene Scanning Software was used to perform a futher analysis results. Conclusions This new HRM method enabled the identification of Leptospira strains at the species and subspecies levels and support the direct genotyping of Leptospira in biological samples without requiring cultures.en_NZ
dc.language.isoenen_NZ
dc.publisherAuckland University of Technology
dc.subjectLeptospirosisen_NZ
dc.subjectLeptospiraen_NZ
dc.subjectHigh Resolution Meltingen_NZ
dc.subjectGenotypingen_NZ
dc.titleUse of high resolution melting for genotyping Leptospira spp.en_NZ
dc.typeThesisen_NZ
thesis.degree.grantorAuckland University of Technology
thesis.degree.levelMasters Theses
thesis.degree.nameMaster of Scienceen_NZ
dc.rights.accessrightsOpenAccess
dc.date.updated2018-05-23T03:05:35Z


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